Distribution of the H1 histone subfractions in Syrian hamster chromatin fractions.
نویسندگان
چکیده
Chromatin from two Syrian hamster tissues: the Kirkman-Robbins hepatoma and the liver, has been separated into soluble (S) and insoluble (P) fractions. Both fractions contain the complete set of five main histones but differ in respect of H1 subfractions. The hepatoma chromatin is known to contain an unusual H1 subfraction, H1 slow [12, 13], probably identical with a similar subfraction present in hamster testes. The content of H1 slow in total H1 histone has been estimated for total, S and P chromatin from hamster hepatoma. The values 20.9 +/- 7.2, 13.8 +/- 1.8 and 26.8 +/- 4.2%, respectively, were obtained.
منابع مشابه
Distribution of the H I Histone Subfractions in Syrian Hamster Chromatin Fractions
Chromatin from two Syrian hamster tissues: the Kirkman-Robbins hepatoma and the liver, has been separated into soluble (S) and insoluble (P) fractions. Both fractions contain the complete set of five main histones but differ in respect of HI subfractions. The hepatoma chromatin is known to contain an unusual HI subfraction, HI slow [12, 13], probably identical with a similar subfration present ...
متن کاملComparative enzymatic degradation of H1 subfractions from Syrian hamster tissues.
An additional hydrolysis site recognized by thrombin on histone H1 molecules was found. Snakes venom proteases from Agkistrodon rhodostoma, Bothrops marajoensis and Bothrops moojeni were further used for the analysis of H1 histones. The presence of the main cleavage site on H1 histone molecules has been established. This site is localized on main N-terminal thrombin peptide. The main venom prot...
متن کاملA low-electrophoretic-mobility H1 histone subfraction from Kirkman-Robbins hamster hepatoma.
A protein showing lower electrophoretic mobility in acidic urea polyacrylamide gels than did the usual histone H1 subfractions has been detected among the H1 histones extracted from chromatin of a transplantable hamster hepatoma, originally induced by Kirkman and Robbins. It was proved to be a true H1 histone subfraction. It differs from the remaining ones by the total chain length, amino acid ...
متن کاملAsynchronous appearance of newly synthesized histone H1 subfractions in HeLa chromatin
Incorporation of radioactive alanine into chromatin-bound subfractions of H1 histone was studied in HeLa cells synchronized by the double thymidine block technique. The subfractions were resolved into three chromatographic peaks by Biorex-70. In the period 5-7 h after release from the thymidine block, peaks I and III showed twice as much incorporation as they did in the period 1-3 h after relea...
متن کاملDoes high-mobility-group non-histone protein HMG 1 interact specifically with histone H1 subfractions?
The interaction of the non-histone chromosomal protein HMG (high-mobility group) 1 with histone H1 subfractions was investigated by equilibrium sedimentation and n.m.r. sectroscopy. In contrast with a previous report [Smerdon & Isenberg (1976) Biochemistry 15, 4242--4247], it was found, by using equilibrium-sedimentation analysis, that protein HMG 1 binds to all three histone H1 subfractions CT...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Zeitschrift fur Naturforschung. Section C, Biosciences
دوره 39 9-10 شماره
صفحات -
تاریخ انتشار 1984